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jove.com
Video: Real Time RT-PCR
62.8K Views. Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two ...
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Reverse transcription polymerase chain reaction RT-PCR Explained
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